![]() ![]() (2021) DNA metabarcoding and morphological methods show complementary patterns in the metacommunity organization of lentic epiphytic diatoms. Sci Rep 10(1):13947.ĭiatom DNA amplification with degenerate primers for Illumina sequencing Lee JW, Lee MW, Ha JS (2020) Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp. ![]() Restriction enzyme site-directed amplification PCR (REDA-PCR) and cloning with microalga DNA (2021) Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: overview and recommendations. Metabarcoding of sedimentary ancient DNA samplesĬapo E, Giguet-Covex C, Rouillard A et al. (2021) Monitoring and contamination incidence of gnotobiotic experiments performed in microisolator cages. Ion Torrent sequencing of bacterial 16S rRNA genesīasic M, Bolsega S, Smoczek A et al. dDuring manufacturing of Platinum II Taq Hot-Start DNA Polymerase, strict measures are taken to control and verify by qPCR that no more than one copy of residual bacterial genomic DNA is present per unit of the polymerase. c Green buffer available as separate item for use with stand-alone enzyme for direct loading gel. bDirect gel loading with green buffer options. Benchtop stability of assembled PCR reactionsĬertified low level of residual human and bacterial DNA dĪThe extension step can be extended up to 60 sec/kb without the effect on specificity.
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